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Product Name
Human GRK6 (His & GST Tag) recombinant protein
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Description
Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).
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Protein name
G protein-coupled receptor kinase 6
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Protein short names
GPRK6; FLJ32135; GRK6
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Uniprot ID
P43250
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Gene Name
GRK6; GPRK6
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human GRK6 isofrom B (P43250-2) (Met 1-Arg 589) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human GRK6/GST chimera consists of 826 amino acids and has a calculated molecular mass of 95.1 kDa. It migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 93 % as determined by SDS-PAGE
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Activity
The specific activity was determined to be 7 nmol/min/mg using casein as substrate.
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Validations
Human GRK6 / GPRK6 Protein (His & GST Tag) SDS-PAGE
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