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Product Name
Follistatin antibody
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Description
Follistatin Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HeLa cells, human skeletal muscle tissue, mouse liver tissue. Positive IP detected in mouse liver tissue. Positive IF detected in mouse olfactory epithelium tissue. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 32-35 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Activin binding protein antibody; follistatin antibody; FST antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Follistatin recombinant protein (Accession Number: NM_013409). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: N/A
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:110742(FST antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:110742(FST antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:110742(FST antibody) at dilution of 1:100 (under 40x lens)
Fluorescent IHC on 1%PLP fixed frozen mouse olfactory epithelium tissue of FST antibody (Catalog No:110742, 1:100). Interesting lamina propria cell stained below the olfactory epithelium. By Dr. Brian Lin (Schwob Lab).
IP Result of anti-FST (IP:Catalog No:110742, 4ug; Detection:Catalog No:110742 1:1000) with mouse liver tissue lysate 6400ug.
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Background
Follistatin (FST) is a member of the tissue growth factor β family and is a secreted glycoprotein that antagonizes many members of the family, including activin A, growth differentiation factor11 , and myostatin. It binds activin A with high affinity and whose expression can be induced by activin A and several other pro-inflammatory cytokines. Activin A-follistatin complexes are biologically inactive and bind to cell surface heparan sulphate-containing proteoglycans for internalisation and degradation. It has also been found to play a significant role in the management of skeletal muscle size and mass. It also has important roles in early embryonic development, differentiation of ovarian granulosa cells, liver fibrosis and polycystic ovarian syndrome.
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References
- Wang G, Burczynski FJ, Hasinoff BB, Zhang K, Lu Q, Anderson JE. Development of a nitric oxide-releasing analogue of the muscle relaxant guaifenesin for skeletal muscle satellite cell myogenesis. Molecular pharmaceutics. 6(3):895-904. 2009.
- Zhang DF, Li XG, Su LJ, Meng QL. Expression of activin A and follistatin in glioblastoma and their effects on U87 in vitro. The Journal of international medical research. 38(4):1343-53. 2010.
- Chen L, Zhang W, Liang HF. Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells. Cell communication and signaling : CCS. 12:18. 2014.
- Ahlfeld SK, Wang J, Gao Y, Snider P, Conway SJ. Initial Suppression of Transforming Growth Factor-β Signaling and Loss of TGFBI Causes Early Alveolar Structural Defects Resulting in Bronchopulmonary Dysplasia. The American journal of pathology. 2016.
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