Flg22 peptide
Not For Human Use, Lab Use Only.
Cat.#: 319503
Special Price 186.9 USD
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Product Name
Flg22 peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
H-TRLSSGLKINSAKDDAAGLQIA-OH
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Sequence
H-Thr-Arg-Leu-Ser-Ser-Gly-Leu-Lys-Ile-Asn-Ser-Ala-Lys-Asp-Asp-Ala-Ala-Gly-Leu-Gln-Ile-Ala-OH
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Length (aa)
22
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Peptide Purity (HPLC)
95.43%
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Molecular Formula
C94H165N29O33
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Molecular Weight
2229.48
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Source
Synthetic
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Form
Powder
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Description
The best-characterized PAMP(pathogen-associated molecular pattern) known to activate innate immunity in plants is flagellin from Pseudomonas bacteria. A conserved N-terminal peptide of flagellin, flg22, is a potent elicitor of defense responses in tomato and Arabidopsis. In Arabidopsis, flg22 is perceived by FLS2, a receptor-like kinase that activates downstream events through a MAP kinase cascade. Pretreatment of Arabidopsis with flg22 peptide not only globally induces defense gene expression, but also protects plants from subsequent infection of the virulent DC3000.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
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References
- Li X, Lin H, Zhang W, Zou Y, Zhang J, Tang X, Zhou JM. Flagellin induces innate immunity in nonhost interactions that is suppressed by Pseudomonas syringae effectors. Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12990-5. doi: 10.1073/pnas.0502425102. Epub 2005 Aug 25. PMID: 16123135; PMCID: PMC1200263.
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
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• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"