FGF7p peptide

FGF7p peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-YASAKWTHNGGEMFVALNQ-OH

H-Tyr-Ala-Ser-Ala-Lys-Trp-Thr-His-Asn-Gly-Gly-Glu-Met-Phe-Val-Ala-Leu-Asn-Gln-OH

19

95.23%

C₉₅H₁₃₈N₂₆O₂₈S

2124.32

Synthetic

Powder

FGF7p is a peptide derived from the sequence of fibroblast growth factor 7 (FGF7) that mimics the biological effects of full‐length FGF7. Similar to FGF7, FGF7p drives downstream signaling in urothelium and urothelial cytoprotection, probably through AKT. FGF7p blocks bladder urothelial apoptosis via AKT and its targets, again similarly to FGF7, and mitigates bladder urothelial injury from cyclophosphamide.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Narla et al (2022) FGF7 peptide (FGF7p) mimetic mitigates bladder urothelial injury from cyclophosphamide. Physiol Rep. 10(7) e15241 PMID: 35388988
• Narla et al (2022) Durability of and role of AKT in FGF7p urothelial protection against cyclophosphamide. Physiol Rep. 10(12) e15358 PMID: 35748317

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.