Ferret CD20/MS4A1 (His Tag) recombinant protein

Ferret CD20/MS4A1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

CD20 (membrane-spanning 4-domains, subfamily A, member 1), also known as MS4A1, is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 / MS4A1 is expressed on all stages of B cell development except the first and last. CD20 / MS4A1 is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells. It is a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. CD20 / MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5(CVID5). CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.

AA960661; MS4A2; BP35; CVID5; MS4A1; LEU-16; CD20; MGC3969; LY-44; S7; B1

Human Cells

A DNA sequence encoding the C-terminal cytoplasmic domain of Ferret (Mustela putorius furo) CD20 (Glu 213-Pro 297) was expressed, with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

Ferret

The recombinant Ferret CD20 consists of 96 amino acids and has a calculated molecular mass of 11.3 kDa. The apparent molecular mass of the recombinant protein is approximately 25 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 90 % as determined by SDS-PAGE