Feleucin-BO1 peptide

Feleucin-BO1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-FLGLLGSLL-NH2

H-Phe-Leu-Gly-Leu-Leu-Gly-Ser-Leu-Leu-NH2

9

95.59%

C₄₆H₇₈N₁₀O₁₀

931.17

Synthetic

Powder

Feleucin-BO1 was found in skin secretion, Oriental fire-bellied toad, Bombina orientalis, Asia.
Feleucin-BO1 is active against S. aureus (MIC 34 uM), but not Gram-negative bacteria or yeast.
Changes of residues G3, G6, and S7 to K increase peptide activity as well as activity spectrum. Changes of G3, G6, and S7 to lysines (i.e. feleucin-K3) enhance the antibacterial activity (MIC 7-14 uM) of the peptide.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Hou X, Du Q, Li R, et al. Feleucin-BO1: a novel antimicrobial non-apeptide amide from the skin secretion of the toad, Bombina orientalis, and design of a potent broad-spectrum synthetic analogue, feleucin-K3. Chem Biol Drug Des. 2015;85(3):259-67.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.