F9170 peptide

F9170 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-GWEALKYLWNLLQYW-OH

H-Gly-Trp-Glu-Ala-Leu-Lys-Tyr-Leu-Trp-Asn-Leu-Leu-Gln-Tyr-Trp-OH

15

95.08%

C₁₀₀H₁₃₅N₂₁O₂₂

1983.26

Synthetic

Powder

F9170 is a peptide identified from the cytoplasmic region of the HIV-1 envelope glycoprotein (Env, gp120-gp41) which is able to inactivate HIV-1 virions and induce necrosis of HIV-1 infected cells. F9170 can enter the brain and lymph nodes and specifically targets the conserved cytoplasmic tail of HIV-1 Env to disrupt the integrity of the viral membrane. F9170 short term administration can control viral loads to below the limit of detection in chronically SHIV infected macaques.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Wang et al (2020) An amphipathic peptide targeting the gp41 cytoplasmic tail kills HIV-1 virions and infected cells. Sci Transl Med. 12(546):eaaz2254 PMID: 32493792

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.