F8K peptide

F8K peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-FEFKFEFKK-OH

H-Phe-Glu-Phe-Lys-Phe-Glu-Phe-Lys-Lys-OH

9

95.03%

C₆₄H₈₈N₁₂O₁₄

1249.45

Synthetic

Powder

F8K is a peptide sequence designed to self-assemble into a β-sheet nanofiber structure, forming a peptide hydrogel. It is one of the self-assembling peptide hydrogels (SAPHs) that have shown promise in supporting the culture of various cell lineages for stem cell culture and tissue engineering applications. F8K is composed of the amino acids phenylalanine (F), glutamic acid (E), and lysine (K). It has a medium net positive charge at pH 7, with a net charge of 1. F8K has been found to have improved RNA-peptide fibril interactions compared to the neutral peptide F8, suggesting that it facilitates RNA binding to the matrix. However, F8K is not so positively charged that it is prevented from filtering through the spin column, unlike other peptides such as FK and KF8K.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Chiesa I, Ligorio C, Bonatti AF, De Acutis A, Smith AM, Saiani A, Vozzi G, De Maria C. Modeling the Three-Dimensional Bioprinting Process of β-Sheet Self-Assembling Peptide Hydrogel Scaffolds. Front Med Technol. 2020 Oct 15;2:571626. doi: 10.3389/fmedt.2020.571626. PMID: 35047879; PMCID: PMC8757771.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.