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Product Name
Eribulin Monoclonal Antibody
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Description
Product has been tested in direct ELISA.
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Tested applications
ELISA
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Species reactivity
Chemical
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Isotype
Mouse IgG1, κ
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Preparation
Eribulin conjugated with BSA. Protein G affinity purified
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Clonality
Monoclonal
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Formulation
PBS(pH7.4), lyophilized. Reconstitution: Adding sterile water, prepare a stock solution of 2.0 mg/ml.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
ELISA: 1:5,000-1:20,000
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Validations
ELISA Binding Assay of Anti-Eribulin Antibody to different proteins.
ELISA analysis of Anti-Eribulin Monoclonal Antibody with Eribulin-BSA, Eribulin-OVA , BAS and OVA. Anti-Eribulin Monoclonal Antibody can bind to Eribulin-BSA or Eribulin-OVA but not carrier proteins.
ELISA Binding Assay of Anti-Eribulin Antibody
Immobilized Eribulin ADC at 2μg/mL (100μL/well) can bind anti-Eribulin mouse monoclonal antibody.
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Background
Eribulin is a synthetic analogue of halichondrin B which is a large polyether macrolide derived from a very strong natural mitotic tubule inhibitor. Eribulin acts by inhibiting the dynamics of the microtubules. It binds to the plus ends of the microtubules and suppresses the microtubule growth in the interphase cells without affecting the shortening phase and sequesters tubulin into nonproductive aggregates, leading to G2/M cell-cycle block and ultimately apoptosis after prolonged mitotic blockage. Its mechanism is distinct from other microtubule inhibitors like vinca alkaloids and taxanes, which affect both the shortening and growing phases.
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References
- Watanabe K, Yui Y, Sasagawa S, Suzuki K, Kanamori M, Yasuda T, Kimura T. Low-dose eribulin reduces lung metastasis of osteosarcoma in vitro and in vivo. Oncotarget. 2019 Jan 4;10(2):161-174. doi: 10.18632/oncotarget.26536. PMID: 30719211; PMCID: PMC6349434.
- Okouneva T, Azarenko O, Wilson L, Littlefield BA, Jordan MA. Inhibition of centromere dynamics by eribulin (E7389) during mitotic metaphase. Mol Cancer Ther. 2008 Jul;7(7):2003-11. doi: 10.1158/1535-7163.MCT-08-0095. PMID: 18645010; PMCID: PMC2562299.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"