Rat Ephrin-A5/EFNA5 (Fc Tag) recombinant protein

Rat Ephrin-A5/EFNA5 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion (By similarity).

Ephrin-A5

EPLG7; EFL-5; AF1; EPL7; RAGS; AL-1; EFNA5; EFL5; LERK7; AV158822; EPHRIN-A5; GLC1M; LERK-7

P97605

Efna5; Eplg7; Lerk7

Human Cells

A DNA sequence encoding the mature form of rat EFNA5 (P97605) (Met 1-Glu 202) was fused with the Fc region of human IgG1 at the C-terminus.

Rat

The recombinant rat EFNA5/Fc is a disulfide-linked homodimer. The reduced monomer comprises 423 amino acids and predicts a molecular mass of 48 kDa. The apparent molecular mass of the rat EFNA5/Fc monomer is approximately 55 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized recombinant Rat EPHA4 Protein (His Tag) (Cat: 503607) at 10 µg/mL (100 μl/well) can bind Recombinant rat EFNA5-Fc (Cat: 502973) with a linear range of 7.8-12.5 ng/ml.