EBOV EBOV-G / Glycoprotein (aa:Met1-Asp320, His Tag)

EBOV EBOV-G / Glycoprotein (aa:Met1-Asp320, His Tag)

• DataSheet
• Material Safety Data Sheets (MSDS)

GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. Binding to CD209 and CLEC4M, which are respectively found on dendritic cells (DCs), and on endothelial cells of liver sinusoids and lymph node sinuses, facilitate infection of macrophages and endothelial cells. These interactions not only facilitate virus cell entry, but also allow capture of viral particles by DCs and subsequent transmission to susceptible cells without DCs infection (trans infection). Binding to the macrophage specific lectin CLEC10A also seems to enhance virus infectivity. Interaction with FOLR1/folate receptor alpha may be a cofactor for virus entry in some cell types, although results are contradictory. After internalization of the virus into the endosomes of the host cell, proteolysis of GP1 by two cysteine proteases, CTSB/cathepsin B and CTSL/cathepsin L presumably induces a conformational change of GP2, unmasking its fusion peptide and initiating membranes fusion (By similarity).

Envelope glycoprotein

GP

Q7T9D9

GP

Human Cells

A DNA sequence encoding Sudan ebola virus (strain Uganda-00) GP (Q7T9D9) (Met1-Asp320) was expressed with a C-terminal polyhistidine tag.

EBOV

The recombinant Sudan ebola virus (strain Uganda-00) GP comprises 299 amino acids and has a predicted molecular mass of 33.8 kDa. The apparent molecular mass of the protein is approximately 46-64 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE