Egg-laying hormone(30-36) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Egg-laying hormone (ELH) is a neuropeptide precursor in the marine mollusk Aplysia. The prohormone undergoes post-translational proteolytic processing, which includes cleavage at Leu-Leu residues, to generate multiple peptide products. Among these, the acidic peptide (AP) is further processed to yield specific fragments such as AP_9–27 and AP_1–20. These peptides are colocalized in vesicles with ELH and are transported to specific neuronal targets, where they are released in a calcium-dependent manner. The processing results in a differential distribution of peptide forms at target cells, and variations such as [Thr²¹]AP can occur.

• Garden R.W., Shippy S.A., Li L., Moroz T.P., Sweedler J.V.; Proteolytic processing of the Aplysia egg-laying hormone prohormone.; Proc. Natl. Acad. Sci. U.S.A. 95:3972-3977(1998).

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.