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Product Name
EGFR (N-terminal) antibody
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Description
EGFR (N-terminal) Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human lung cancer tissue. Positive FC detected in HepG2 cells. Positive WB detected in A431 cells. Observed molecular weight by Western-blot: 145 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human; other species not tested.
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Alternative names
EGFR antibody; ERBB antibody; ERBB1 antibody; HER1 antibody; mENA antibody; PIG61 antibody; Proto oncogene c ErbB 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EGFR (N-terminal) recombinant protein (Accession Number: NM_001346897). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:110217(EGFR Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:110217(EGFR Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:110217(EGFR Antibody) at dilution of 1:50
1X10^6 HepG2 cells were stained with 0.2ug EGFR antibody (Catalog No:110217, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
ERBB1, also mamed as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody can recognize all the isoforms.
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References
- Niu C, Liu N, Liu J. Vitamin A maintains the airway epithelium in a murine model of asthma by suppressing glucocorticoid-induced leucine zipper. Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. 2015.
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