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Product Name
Human ENTPD3 (His Tag) recombinant protein
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Description
Has a threefold preference for the hydrolysis of ATP over ADP.
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Protein name
Ectonucleoside triphosphate diphosphohydrolase 3
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Protein short names
ECTO-ATPASE 3; ECTO-ATP DIPHOSPHOHYDROLASE 3; ECTO-APYRASE 3; CD39L3; HB6; CD39-LIKE 3; NTPDASE-3; CD39 ANTIGEN-LIKE 3; NTPDASE 3; ECTO-ATPDASE 3
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Uniprot ID
O75355
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Gene Name
ENTPD3; CD39L3
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human ENTPD3 (O75355) extracellular domain (Gln 44-Pro 485) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human ENTPD3 (aa44-485) consists of 452 amino acids and predicts a molecular mass of 51 kDa. It migrates as an approximately 50 KDa band in SDS-PAGE under reducing conditions.
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Purity
> 97 % as determined by SDS-PAGE
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Activity
Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >70,000 pmol/min/µg. -
Validations
Human ENTPD3 / NTPDase3 / CD39L3 Protein (His Tag) SDS-PAGE
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