Ecdysis-triggering hormone, Drm-ETH-1 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Ecdysis-triggering hormone (ETH) is a neuropeptide that plays a critical role in the regulation of ecdysis, the process of molting, in insects. In Drosophila melanogaster, ETH is released by Inka cells located on the tracheal tubes. This hormone directly and sequentially activates a cascade of neuropeptides within the central nervous system to initiate the precise behavioral sequence of ecdysis. The study demonstrates that incubation of larval central nervous system tissue in an ETH solution can prematurely induce the expression of neuropeptides such as CCAP and Bursicon in specific efferent neurons, specifically overcoming the temporal repression mediated by the Hox gene Abdominal-B.

The research indicates that ETH functions as a key signaling molecule within the endocrine system to coordinate the physiological and behavioral events required for successful shedding of the old cuticle. Its action is integrated with developmental hormonal cues, such as the ecdysone pulse in late larval stages, which counteracts repressive genetic mechanisms to permit the terminal differentiation of neurons essential for the ecdysis program.

• Wegener, C., Reinl, T., Jänsch, L. and Predel, R. (2006), Direct mass spectrometric peptide profiling and fragmentation of larval peptide hormone release sites in Drosophila melanogaster reveals tagma-specific peptide expression and differential processing. Journal of Neurochemistry, 96: 1362-1374.
• Moris-Sanz M, Estacio-Gómez A, Sánchez-Herrero E, Díaz-Benjumea FJ. The study of the Bithorax-complex genes in patterning CCAP neurons reveals a temporal control of neuronal differentiation by Abd-B. Biol Open. 2015 Aug 14;4(9):1132-42.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.