EcDBS1R5 peptide

EcDBS1R5 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

1 Vial

H-PMKKLKLALRLAAKIAPVW-OH

H-Pro-Met-Lys-Lys-Leu-Lys-Leu-Ala-Leu-Arg-Leu-Ala-Ala-Lys-Ile-Ala-Pro-Val-Trp-OH

19

95.78

C₁₀₃H₁₇₉N₂₇O₂₀S

2147.75

Synthetic

The computationally generated peptide, EcDBS1R5 (PMKKLKLALRLAAKIAPVW), inspired by an E. coli AMP, was active at 8 μmol L−1 against P. aeruginosa in in vitro assays, while in a skin scarification mouse model infected by P. aeruginosa, the same peptide was active only at a concentration 8-fold higher (64 μmol L−1), even though the concentration was lower than its cytotoxic activity (>128 μmol L−1).

Ideally EcDBS1R5 peptide should be stored in a freezer at or below -9C. EcDBS1R5 peptide should be refrigerated after reconstitution. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Cardoso, M. H., Cândido, E. S., Chan, L. Y., Der Torossian Torres, M., Oshiro, K. G. N., Rezende, S. B., Porto, W. F., Lu, T. K., de la Fuente-Nunez, C., Craik, D. J., and Franco, O. L. A (2018) Computationally Designed Peptide Derived from Escherichia coli as a Potential Drug Template for Antibacterial and Antibiofilm Therapies. ACS Infect. Dis. 4, 1727−1736.

Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.

TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.

TFA Removal Service is recommended for:

• Peptides that will be used in cellular assays
• Peptides that will be used as APIs or in manufactured products
• For hydrophilic peptides containing numerous basic residues