eCATH-2 peptide

eCATH-2 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

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Lys-Arg-Arg-His-Trp-Phe-Pro-Leu-Ser-Phe-Gln-Glu-Phe-Leu-Glu-Gln-Leu-Arg-Arg-Phe-Arg-Asp-Gln-Leu-Pro-Phe-Pro

27

95.8%

Synthetic

Powder

present in uncleaved forms in horse neutrophils. active against a broad range of bacteria such as E. coli ATCC 25922, E. coli ML 35 (MIC 14 ug/ml), S. marcescens ATCC 8100, K. pneumoniae ATCC 13883 (MIC 28.5), S. aureus ATCC 25923 (MIC 57 ug/ml), S. epidermidis ATCC 12228 (MIC 28.5 ug/ml), and B. megaterium Bm11 (MIC 14 ug/ml). Active against fungi various C. neoformans, R. rubra (MIC 21-57 ug/ml) in RPMI medium (Skerlavaj B et al., 2001). Updated 7/8/2013. eCATH-2 has Antimicrobial activity. The source of eCATH-2 is myeloid cells, Horse Equus asinus.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Scocchi M, Bontempo D, Boscolo S, Tomasinsig L, Giulotto E, Zanetti M. Novel cathelicidins in horse leukocytes(1). FEBS Lett. 1999;457(3):459-64.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.