eCATH-1 peptide

eCATH-1 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

KRFGRLAKSFLRMRILLPRRKILLAS

Lys-Arg-Phe-Gly-Arg-Leu-Ala-Lys-Ser-Phe-Leu-Arg-Met-Arg-Ile-Leu-Leu-Pro-Arg-Arg-Lys-Ile-Leu-Leu-Ala-Ser

26

96.8%

31409

Synthetic

Powder

Potently active against a broad range of bacteria such as E. coli ATCC 25922, E. coli ML 35, P. aeruginosa ATCC 27853, S. marcescens ATCC 8100, K. pneumoniae ATCC 13883, S. aureus ATCC 25923, MRSA, S. epidermidis ATCC 12228, S. equinus, and B. megaterium Bm11 (MIC 3-50 ug/ml). Active also against fungi various C. neoformans, R. rubra (MIC 3-12.5 ug/ml) in RPMI medium and Sabouraud medium (50 ug/ml for R. rubra in Sabouraud medium) (Skerlavaj B et al., 2001). Animal model:mouse, the equine antimicrobial peptide eCATH1 is effective against the facultative intracellular pathogen Rhodococcus equi (R. equi) in mice (Schlusselhuber M et al., 2013). Updated 7/8/2013. eCATH-1 has Antimicrobial activity. eCATH-1 was found in Horse Equus asinus.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Scocchi M, Bontempo D, Boscolo S, Tomasinsig L, Giulotto E, Zanetti M. Novel cathelicidins in horse leukocytes(1). FEBS Lett. 1999;457(3):459-64.
• Cauchard S, Van Reet N, Büscher P, Goux D, Grötzinger J, Leippe M, Cattoir V, Laugier C, Cauchard J. Killing of Trypanozoon Parasites by the Equine Cathelicidin eCATH1. Antimicrob Agents Chemother. 2016 Apr 22;60(5):2610-9.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.