EBOV EBOV-G / Glycoprotein (Fc Tag)

EBOV EBOV-G / Glycoprotein (Fc Tag)

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The fourth gene of the EBOV genome encodes a 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD); the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, ?-integrins, folate receptor-?, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry; the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.

Human Cells

A DNA sequence encoding the Zaire ebolavirus(strain H.sapiens-wt/GIN/2014/Kissidougou-C15) GP (AHX24649.1) (Glu502-Gln650) was expressed with the Fc region of mouse IgG1 at the N-terminus.

EBOV

The recombinant Zaire ebolavirus(strain H.sapiens-wt/GIN/2014/Kissidougou-C15) GP consists 383 amino acids and predicts a molecular mass of 43.4 kDa.

> 90 % as determined by SDS-PAGE.