E70K peptide

E70K peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Ac-KENWVQRVVEKFLKRAKNS-NH2

Ac-Lys-Glu-Asn-Trp-Val-Gln-Arg-Val-Val-Glu-Lys-Phe-Leu-Lys-Arg-Ala-Lys-Asn-Ser-NH2

19

95.48%

C₁₀₈H₁₇₈N₃₄O₂₈

2400.77

Synthetic

Powder

E70K, also known as peptide 2, is the C-terminal CXCL8 peptide fragment CXCL8 (54-72), with a glutamic acid (E) substituted at position 70 with lysine (K) to increase positive charge. Similarly to CXCL8 (54-72), E70K inhibits CXCL8-induced neutrophil transendothelial migration and provides a lead for further development of therapeutic inhibitors of neutrophil-mediated inflammation based on modulation of chemokine–GAG binding.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Martínez-Burgo et al (2019), A C-terminal CXCL 8 peptide based on chemokine-glycosaminoglycan interactions reduces neutrophil adhesion and migration during inflammation. Immunol. 157 173 PMID: 31013364

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.