Human Cbl-c / CBL-3 (His & GST Tag) recombinant protein

Human Cbl-c / CBL-3 (His & GST Tag) recombinant protein

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Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Functionally coupled with the E2 ubiquitin-protein ligases UB2D1, UB2D2 and UB2D3. Regulator of EGFR mediated signal transduction; upon EGF activation, ubiquitinates EGFR. Isoform 1, but not isoform 2, inhibits EGF stimulated MAPK1 activation. Promotes ubiquitination of SRC phosphorylated at 'Tyr-419'. In collaboration with CD2AP may act as regulatory checkpoint for Ret signaling by modulating the rate of RET degradation after ligand activation; CD2AP converts it from an inhibitor to a promoter of RET degradation; the function limits the potency of GDNF on neuronal survival.

E3 ubiquitin-protein ligase CBL-C

E3 UBIQUITIN PROTEIN LIGASE C; CASITAS B-LINEAGE LYMPHOMA C; CBLC; 2310076I21RIK; SH3-BINDING PROTEIN CBL-3; RING FINGER PROTEIN 57; RNF57; SH3-BINDING PROTEIN CBL-C; SIGNAL TRANSDUCTION PROTEIN CBL-C; CBL3; CAS-BR-M (MURINE) ECTROPIC RETROVIRAL TRANSFORMING SEQUENCE C; CBL-3; 2310079L19RIK; CBL PROTO-ONCOGENE; CAS-BR-M (MURINE) ECOTROPIC RETROVIRAL TRANSFORMING SEQUENCE C; CBL-SL

Q9ULV8

CBLC; CBL3; RNF57

Baculovirus-Insect Cells

A DNA sequence encoding the human CBLC (AAH28915.1) (Met1-Ala474) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human CBLC chimera consists of 711 amino acids and has a calculated molecular mass of 80.2 kDa. The recombinant protein migrates approximately 66 kDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE