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Product Name
E.coli trpB/b1261/JW1253 recombinant protein
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Description
Tryptophan synthase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan. It is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from animals such as humans. Tryptophan synthase typically exists as an α-ββ-α complex.The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate: L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.The beta subunits catalyze the irreversible condensation of indole and serine to form tryptophan in a pyridoxal phosphate (PLP) dependent reaction. Their assembly into a complex leads to structural changes in both subunits resulting in reciprocal activation.
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Protein name
tryptophan synthase, beta subunit(trpB)
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Protein short names
b1261; JW1253
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Uniprot ID
P0A879
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the E.coli trpB/b1261/JW1253 Thr2-Ile397 is expressed with a 6His tag at the N-terminus.
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Protein Species
E.coli
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Purity
>95% as determined by SDS-PAGE.
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Validations
E.coli trpB/b1261/JW1253 recombinant protein
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