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Product Name
E. coli Enoyl-ACP Reductase/FABI (His Tag) recombinant protein
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Description
Enoyl-ACP reductase, also known as NADH-dependent enoyl-ACP reductase and FABI, is a cell inner membrane and peripheral membrane protein which belongs to the short-chain dehydrogenases/reductases (SDR) family and FabI subfamily. Microorganisms produce many kinds of antibiotics which function in an antagonistic capacity in nature where they have much competition. Bacterial FAS provides essential fatty acids for use in the assembly of key cellular components. Among them, FABI is an enoyl-ACP reductase which catalyzes the final and rate-limiting step of bacterial FAS. The antibiotic diazaborine interferes with the activity by binding to the protein. FABI is a potential target for selective antibacterial action, because it shows low overall sequence homology with mammalian enzymes. Various compounds have been reported as inhibitors of bacterial FabI-inhibitory compounds.
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Protein short names
ENOYL-ACP REDUCTASE; ENVM; ESCHERICHIA COLI STR. K-12 SUBSTR. MG1655; FABI; JW1281; B1288; ECK1283; GTS; QMEA
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the E.coli FABI (NP_287908.1) (Gly 2-Lys 262) was expressed, with a polyhistide tag at the N-terminus.
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Protein Species
E. coli
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Molecular weight
The recombinant E.coli FABI consisting of 276 amino acids and has a calculated molecular mass of 29.5 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 98 % as determined by SDS-PAGE
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Validations
E.coli Enoyl-ACP Reductase / FABI Protein (His Tag) SDS-PAGE
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