Drosocin peptide

Drosocin peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

GKPRPYSPRPTSHPRPIRV

Gly-Lys-Pro-Arg-Pro-Tyr-Ser-Pro-Arg-Pro-Thr-Ser-His-Pro-Arg-Pro-Ile-Arg-Val

19

97%

C₉₈H₁₆₀N₃₄O₂₄

2198.52

Synthetic

Powder

It is induced via the IMD pathway. Position T11 is o-glycosylated and important for full activity. Subsequent analysis showed that S7 may also be glycosylated. Turn elements occur near residues 4-7, 10-13, 17, and 18. At modorate concentrations, this peptide, as well as pyrrhocoricin (AP170), is also active against a few Gram-positive bacteria such as B. megaterium (44% homology to E. coli DnaK D-E helix) and M. luteus (Hoffmann R et al. 1999 Biochim Biophys Acta 1426:459-67). Mechanism of Action (MOA): It binds to an internal protein target DnaK (Eur J Biochem 2002; 269: 4226-4237). Note that all D-drosocin is inactive (so a non-membrane target) and the L form kills bacteria in hours not in minutes. Updated 12/2012 GW. Drosocin has Antibacterial activity. The source of Drosocin is Fruit fly, Drosophila melanogaster.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Bulet P, Dimarcq JL, Hetru C, et al. A novel inducible antibacterial peptide of Drosophila carries an O-glycosylated substitution. J Biol Chem. 1993;268(20):14893-7.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.