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Product Name
DOPA decarboxylase antibody
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Description
DOPA decarboxylase Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue. Positive WB detected in mouse brain tissue, mouse kidney tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 48-50 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AADC antibody; DDC antibody; DOPA decarboxylase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DOPA decarboxylase recombinant protein (Accession Number: XM_047419931). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110059(DDC antibody) at dilution of 1:1000
IP Result of anti-DDC (IP:Catalog No:110059, 4ug; Detection:Catalog No:110059 1:800) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:110059(DDC Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:110059(DDC Antibody) at dilution of 1:50 (under 40x lens)
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Background
Aromatic-L-amino-acid decarboxylase belongs to the pyridoxal-dependent aminotransferase superfamily.DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD).Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines.
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References
- Ming M, Li X, Fan X. Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson's disease. Journal of translational medicine. 7:53. 2009.
- Fox DA, Hamilton WR, Johnson JE. Gestational lead exposure selectively decreases retinal dopamine amacrine cells and dopamine content in adult mice. Toxicology and applied pharmacology. 256(3):258-67. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"