DLTAGLLE peptide

DLTAGLLE peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-DLTAGLLE-OH

H-Asp-Leu-Thr-Ala-Gly-Leu-Leu-Glu-OH

8

95.99%

C₃₆H₆₂N₈O₁₄

830.92

Synthetic

Powder

DLTAGLLE is a novel ACE-inhibitory peptide which was isolated and purified from Lepidotrigla microptera protein hydrolysate (LMPH) using various techniques such as ultrafiltration, gel filtration chromatography, and preparative high performance liquid chromatography (prep-HPLC). It has been identified as a noncompetitive inhibitor by Lineweaver-Burk plot. The amino acid sequence of the purified peptide is Phe-Leu-Thr-Ala-Gly-Leu-Leu-Asp. The IC50 value of DLTAGLLE was found to be 0.13 mg/mL. The peptide has ACE(Angiotensin Converting Enzyme)-inhibitory activity, which means it can help regulate blood pressure. It is stable across a range of temperatures (<100 • C) and pH values (3.0-11.0) and retained after gastrointestinal digestion. DLTAGLLE has the potential to develop functional food or novel ACE-inhibitor drugs.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Hu X, Dai Z, Jin R. Purification and Identification of a Novel Angiotensin Converting Enzyme Inhibitory Peptide from the Enzymatic Hydrolysate of Lepidotrigla microptera. Foods. 2022 Jun 26;11(13):1889. doi: 10.3390/foods11131889. PMID: 35804705; PMCID: PMC9265830.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.