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Product Name
DIAP1 antibody
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Description
DIAP1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells, Hela cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, COS-7 cells, human heart tissue, human skeletal muscle tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 140-150 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat, Monkey; other species not tested.
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Alternative names
DFNA1 antibody; DIA1 antibody; DIAP1 antibody; DIAPH1 antibody; Diaphanous related formin 1 antibody; DRF1 antibody; FLJ25265 antibody; hDIA1 antibody; LFHL1 antibody; Protein diaphanous homolog 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DIAP1 recombinant protein (Accession Number: BC007411). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109937(DIAP1 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using DIAPH1 antibody Catalog No:109937 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-DIAP1 (IP:Catalog No:109937, 4ug; Detection:Catalog No:109937 1:500) with HeLa cells lysate 1200ug.
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Background
DIAPH1 represents diaphanous homolog 1 (Drosophila)
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References
- Kher SS, Struckhoff AP, Alberts AS, Worthylake RA. A novel role for p115RhoGEF in regulation of epithelial plasticity. PloS one. 9(1):e85409. 2014.
- Pettee KM, Dvorak KM, Nestor-Kalinoski AL, Eisenmann KM. An mDia2/ROCK signaling axis regulates invasive egress from epithelial ovarian cancer spheroids. PloS one. 9(2):e90371. 2014.
- Arden JD, Lavik KI, Rubinic KA. Small-molecule agonists of mammalian Diaphanous-related (mDia) formins reveal an effective glioblastoma anti-invasion strategy. Molecular biology of the cell. 26(21):3704-18. 2015.
- Touré F, Fritz G, Li Q. Formin mDia1 mediates vascular remodeling via integration of oxidative and signal transduction pathways. Circulation research. 110(10):1279-93. 2012.
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