Dhvar4 peptide

Dhvar4 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KRLFKKLLFSLRKY-NH2

H-Lys-Arg-Leu-Phe-Lys-Lys-Leu-Leu-Phe-Ser-Leu-Arg-Lys-Tyr-NH2

14

95.72%

C₉₀H₁₅₁N₂₅O₁₆

1839.31

Synthetic

Powder

Dhvar4 is a derivative of human histatin 5, a member of a family of antifungal and antibacterial histidine-rich proteins secreted from salivary glands. It is fungicidal at low micromole concentration, while its bactericidal activity is relatively weak. The activity of antimicrobial peptide might be enhanced after improving its ability to reach the cell membrane of target micro-organism.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Lu X, Wan L, Yang H, Zhang J, Li S, Kang M, Li Y, Cheng J. Fusion of fungicidal peptide dhvar4 to enterococcal peptide pheromone increases its bactericidal activity against Enterococcus faecalis. Chem Biol Drug Des. 2006 Oct;68(4):220-4. doi: 10.1111/j.1747-0285.2006.00434.x. PMID: 17105486.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.