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Product Name
Recombinant DFFB protein (His tag)
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Description
Recombinant Human DNA Fragmentation Factor Subunit Beta (DFFB) Fragment (1-338) – a high-purity protein encompassing the full CIDE-N domain, designed for studying apoptosis-related DNA fragmentation and chromatin condensation mechanisms.
DNA Fragmentation Factor Subunit Beta (DFFB), also known as Caspase-Activated DNase (CAD), is a nuclease critical for inducing DNA fragmentation and chromatin condensation during apoptosis. It degrades naked DNA and drives the morphological changes characteristic of programmed cell death. The protein is localized in both the cytoplasm and nucleus, facilitating its role in nuclear DNA breakdown upon activation. This recombinant fragment provides researchers with a targeted tool to investigate the specific functions of the CIDE-N domain in apoptotic pathways.
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Protein name
DNA fragmentation factor subunit beta
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Uniprot ID
O76075
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Gene Name
DFFB; CAD; DFF2; DFF40
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-338 aa (O76075) were fused with C-terminal 6His tag.
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Sequence
MLQKPKSVKL RALRSPRKFG VAGRSCQEVL RKGCLRFQLP ERGSRLCLYE DGTELTEDYF PSVPDNAELV LLTLGQAWQG YVSDIRRFLS AFHEPQVGLI QAAQQLLCDE QAPQRQRLLA DLLHNVSQNI AAETRAEDPP WFEGLESRFQ SKSGYLRYSC ESRIRSYLRE VSSYPSTVGA EAQEEFLRVL GSMCQRLRSM QYNGSYFDRG AKGGSRLCTP EGWFSCQGPF DMDSCLSRHS INPYSNRESR ILFSTWNLDH IIEKKRTIIP TLVEAIKEQD GREVDWEYFY GLLFTSENLK LVHIVCHKKT THKLNCDPSR IYKPQTRLKR KQPVRKRQLE HHHHHH
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 40.16 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 41 kDa band.
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Purity
>85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Validations
Recombinant DFFB protein (His tag) SDS-PAGE
M: Marker; Lane 1: DFFB; Lane 2: BSA
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"