Decoralin peptide

Decoralin peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

SLLSLIRKLIT

Ser-Leu-Leu-Ser-Leu-Ile-Arg-Lys-Leu-Ile-Thr

11

95.6%

C₅₈H₁₀₉N₁₅O₁₅

1256.57

Synthetic

Powder

Active against Gram-positive S. aureus CCT 6538, S. saprophyticus, B. subtilis CCT 2471, B. thuringiensis (MIC all at 40 uM), Gram-negative E. coli ATCC 25922 (MIC 80 uM), K. pneumoniae ATCC 13883 (80 uM), and A. faecalis ATCC 8750 (MIC 40 uM), and yeast C. albicans (40 uM). It only showed weak leishmanicidal activity IC50=72 uM (cf. amidated 11 uM). Essentially not hemolytic. Helical in TFE, SDS micelles, or PC/PG (70/30) vesicles, but not in PC vesicles. Sequence validated by Dr. Jan A. Hiss. Updated 5/23/12. Decoralin has Antibacterial, Antifungal activity. Decoralin was found in venom, solitary eumenine wasp, Oreumenes decoratus.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Konno K, Rangel M, Oliveira JS, et al. Decoralin, a novel linear cationic alpha-helical peptide from the venom of the solitary eumenine wasp Oreumenes decoratus. Peptides. 2007;28(12):2320-7.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.