DDDEEKC peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

The DDDEEKC peptide is a bioinspired sequence derived from the N-terminal region of statherin, a salivary acquired pellicle protein. This heptapeptide exhibits a high affinity for hydroxyapatite surfaces due to its anionic character, primarily conferred by the aspartic acid (D) and glutamic acid (E) residues. The adsorption capacity is attributed to the peptide's negative charge density and its potential to adopt specific conformations, such as an α-helical structure, which facilitate strong interactions with the calcium ions in the hydroxyapatite lattice.

Functionally, the DDDEEKC sequence serves as an effective adhesion motif, enabling targeted binding to mineralized substrates like tooth enamel. When conjugated to other molecular entities, such as polyphenol-based compounds like tannic acid, the peptide directs the assembly and localization of the conjugate onto hydroxyapatite. This specific adsorption provides a stable interface for subsequent processes, such as the templated growth of hydroxyapatite crystals from calcium and phosphate ions, mimicking aspects of natural biomineralization.

• Yang X, Yang B, He L, Li R, Liao Y, Zhang S, Yang Y, Xu X, Zhang D, Tan H, Li J, Li J. Bioinspired Peptide-Decorated Tannic Acid for in Situ Remineralization of Tooth Enamel: In Vitro and in Vivo Evaluation. ACS Biomater Sci Eng. 2017 Dec 11;3(12):3553-3562. doi: 10.1021/acsbiomaterials.7b00623. Epub 2017 Oct 23. PMID: 33445390.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.