Danio rerio (zebrafish) VEGF / VEGFA / VEGF165 (zebrafish) recombinant protein

Danio rerio (zebrafish) VEGF / VEGFA / VEGF165 (zebrafish) recombinant protein

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Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.

RP1-261G23.1; VEGF-A; VEGF; VPF; MVCD1; MGC70609

Baculovirus-Insect Cells

A DNA sequence encoding the zebrafish VEGF165 (O73682-Isoform VEGF165) (Met 1-Arg188) was expressed and purified.

Danio rerio (zebrafish)

The recombinant zebrafish VEGF165 consists of 165 amino acids and has a calculated molecular mass of 19.2 kDa. It migrates as an 22 KDa band in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC).
The ED₅₀ for this effect is typically 10-30 ng/mL.