Cynomolgus RBP4 (His Tag) recombinant protein

Cynomolgus RBP4 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM).

RBP4; PRO2222; RDCCAS; RBP; RBPA; RBP-4; OTTHUMP00000020115; PRBP

Human Cells

A DNA sequence encoding the cynomolgus RBP4 (Met1-Leu201) was expressed with a polyhistidine tag at the C-terminus.

Cynomolgus

The recombinant cynomolgus RBP4 comprises 194 amino acids and has a calculated molecular mass of 22.6 KDa. The apparent molecular mass of it is approximately 23 KDa respectively in SDS-PAGE.

> 95 % as determined by SDS-PAGE

Measured by its ability to bind alltrans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4.
The 50% binding concentration (BC50) is >1 µM