Cynomolgus IGFBP4 (His Tag) recombinant protein

Cynomolgus IGFBP4 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Insulin-like growth factor binding protein 4 (IGFBP-4) is a 24-kDa protein that binds insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are clearly distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF, or act in an IGF-independent manner in the prostate. IGF-binding protein-4 (IGFBP-4) inhibits IGF-I action in vitro and is the most abundant IGFBP in the rodent arterial wall. Expression of IGFBP-4 mRNA in nontransgenic littermates was maximal in liver and kidney. IGFBP-4 is a functional antagonist of IGF-I action on SMC. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.

IGFBP4; RP23-333D2.6; AI875747; IGFBP-4; IBP4; DEB2; HT29-IGFBP; BP-4

Human Cells

A DNA sequence encoding the cynomolgus IGFBP4 (Met1-Glu258) was expressed with a polyhistidine tag at the C-terminus.

Cynomolgus

The recombinant heterodimer of cynomolgus IGFBP4 comprises 248 amino acids and has a calculated molecular mass of 27.4 KDa. The apparent molecular mass of the protein is approximately 33-36 KDa respectively in SDS-PAGE.

> 80 % as determined by SDS-PAGE

Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.).
The ED₅₀ for this effect is typically 0.04-0.2 μg/mL in the presence of 14 ng/mL human IGFII.