Cynomolgus EphB6/Eph Receptor B6 (Fc Tag) recombinant protein

Cynomolgus EphB6/Eph Receptor B6 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 is an unusual Eph receptor, lacking catalytic capacity due to alterations in its kinase domain. Interestingly, increased metastatic activity is associated with reduced EphB6 receptor expression in several tumor types, including breast cancer. This emphasizes the potential of EphB6 to act as a suppressor of cancer aggressiveness. EphB6 suppress cancer invasiveness through c-Cbl-dependent signaling, morphologic changes, and cell attachment and indicate that EphB6 may represent a useful prognostic marker and a promising target for therapeutic approaches. EphB6 can both positively and negatively regulate cell adhesion and migration, and suggest that tyrosine phosphorylation of the receptor by an Src family kinase acts as the molecular switch for the functional transition. In addition, Ephrin-B2 may be a physiological ligand for the EphB6 receptor.

MEP; HEP; MGC129911; MGC129910; EPHB6; CEKL

Human Cells

A DNA sequence encoding the cynomolgus EPHB6 (Met1-Ser591) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

Cynomolgus

The recombinant cynomolgus EPHB6 is a disulfide-linked homodimer. The reduced monomer comprises 801 amino acids and has a calculated molecular mass of 86.9 KDa.The apparent molecular mass of the protein is approximately 97 KDa respectively in SDS-PAGE.

> 85 % as determined by SDS-PAGE

1. Measured by its ability to bind human EFNB1-His (Cat:501410) in a functional ELISA.
2. Immobilized EFNB2-His (Cat:503701) at 10 μg/ml (100 μl/well) can bind Cynomolgus EPHB6-Fc, The EC₅₀ of Cynomolgus EPHB6-Fc is 20.6-48 ng/ml.