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Product Name
Cynomolgus CD3D & CD3E recombinant protein
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD3E extracellular domain (Met 1-Asp 117) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD3D extracellular domain (Met 1-Ala 105) with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the CD3E&CD3D heterodimer was purified.
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Protein Species
Cynomolgus
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Molecular weight
The recombinant heterodimer of cynomolgus CD3E&CD3D comprises 679 (346+333) amino acids and has a calculated molecular mass of 76.7 (39.1+ 37.6) KDa. The apparent molecular mass of cyno CD3E&CD3D heterodimer is approximately 52 & 43 KDa respectively in SDS-PAGE.
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Purity
(50.3+48.5) % as determined by SDS-PAGE
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Validations
Cynomolgus CD3D & CD3E Heterodimer Protein SDS-PAGE
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