Cynomolgus CD33 / Siglec-3 (His Tag) recombinant protein

Cynomolgus CD33 / Siglec-3 (His Tag) recombinant protein

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Myeloid cell surface antigen CD33 also known as Sialic acid binding Ig-like lectin 3, CD33 antigen or Siglec-3, is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This Single-pass type I membrane protein contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD33 /Siglec-3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. CD33 /Siglec-3 preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia (in vitro). CD33/Siglec-3 can function as a sialic acid-dependent cell adhesion molecule and that binding can be modulated by endogenous sialoglycoconjugates when CD33 is expressed in a plasma membrane.

GP67; P67; CD33; FLJ00391; SIGLEC3; SIGLEC-3

Human Cells

A DNA sequence encoding the cynomolgus CD33 (XP_005590138.1) (Met1-Gly248) was expressed with a polyhistidine tag at the C-terminus. Cynomolgus and Rhesus CD33 sequences are identical.

Cynomolgus

The recombinant cynomolgus CD33 comprises 244 amino acids and has a calculated molecular mass of 27.3 KDa. The apparent molecular mass of it is approximately 45.2 KDa in SDS-PAGE under reducing condition.

> 95 % as determined by SDS-PAGE