Cynomolgus Autotaxin/ENPP2 (His Tag) recombinant protein

Cynomolgus Autotaxin/ENPP2 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

ENPP2 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2), also referred as Autotaxin, is a secreted enzyme encoded by the ENPP2 gene. This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The Autotaxin protein is important for generating the lipid signaling molecule lysophosphatidic acid (LPA), which is a potent mitogen, which facilitates cell proliferation and migration, neurite retraction, platelet aggregation, smooth muscle contraction, actin stress formation and cytokine and chemokine secretion. ATX has been found to catalyze the formation of cyclic phosphatidic acid (cPA), which have antitumor role by antimitogenic regulation of cell cycle, inhibition of cancer invasion and metastasis. LPA receptors and ATX are upregulated in numerous cancer cell types and show expression patterns that correlate with tumor cell invasiveness. Thus, Autotaxin has recently emerged as an attractive target for the development of anti-cancer chemotherapeutics. In addition, Serum ATX activity was found to be enhanced in relation to hepatic fibrosis in chronic liver disease due to hepatitis virus C infection.

FLJ26803; AUTOTAXIN; NPP2; NPPS2; ATX-X; ATX; ENPP2; PD-IALPHA; PDNP2; LYSOPLD

Human Cells

A DNA sequence encoding the cynomolgus ENPP2 (XP_005564040.1) (Asp49-Ile863) was expressed with a polyhistidine tag at the N-terminus.

Cynomolgus

The recombinant cynomolgus ENPP2 consists 834 amino acids and predicts a molecular mass of 96 kDa.

> 85 % as determined by SDS-PAGE.

Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP).
The specific activity is > 1,000 pmol/min/µg.