cyclo-(D-Trp-Tyr) peptide

cyclo-(D-Trp-Tyr) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Cyclo-(D-Trp-Tyr)

Cyclo-(D-Trp-Tyr)

2

95%

C₂₀H₁₉N₃O₃

349.39

Synthetic

Powder

The cyclo-(d-Trp-Tyr) peptide nanotubes (PNTs) self-associated at concentrations above 0.01 mg/mL. cyclo-(d-Trp-Tyr) PNTs could be used as an oral gene delivery carrier. The results showed that PNTs and genes could distribute to duodenum, stomach, liver and kidney after the first oral PNTs. PNTs complexed with anti-apoptotic plasmid caspase 3 silencing shRNA eye drop delivery decreased 41% of caspase 3 activity after the first dose by caspase 3 activity and Western blot analysis.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Hsieh, W. H., Chang, S. F., Chen, H. M., Chen, J. H., & Liaw, J. (2012). Oral gene delivery with cyclo -(d -Trp-Tyr) peptide nanotubes. Molecular Pharmaceutics, 9(5), 1231-1249. https://doi.org/10.1021/mp200523n

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.