Cyclo(Arg-Gly-Asp-D-Phe-Val) peptide

Cyclo(Arg-Gly-Asp-D-Phe-Val) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

c(RGDfV)

Cyclo(Arg-Gly-Asp-D-Phe-Val)

5

95%

C₂₆H₃₈N₈O₇

574.64

Synthetic

Powder

c(RGDfV) is a potent inhibitor of cell adhesion. It inhibited tumor cell adhesion to laminin and vitronectin substrates. In vivo investigations in rats provided the first evidence of its utility in the amelioration of ischemic acute renal failure. c(RGDfV) inhibited tubular obstruction by preventing cell-cell adhesion. Hammes et al. showed that the cyclopeptide can also be used to prevent retinal neovascularization. c(RGDfV) binds to αvβ3 receptors.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• M. Friedlander, C.L. Theesfeld, M. Sugita, M. Fruttiger, M.A. Thomas, S. Chang, and D.A. Cheresh, Proc. Natl. Acad. Sci. USA, 93, 9764 (1996). (Pharmacol.)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.