CW49 peptide

CW49 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-APFRMGICTTN-OH

H-Ala-Pro-Phe-Arg-Met-Gly-Ile-Cys-Thr-Thr-Asn-OH

11

95.44%

C₅₁H₈₃N₁₅O₁₅S₂

1210.45

Synthetic

Powder

CW49 is a small peptide composed of 11 amino acid residues with the sequence APFRMGICTTN, identified from the skin of the frog Odorrana grahami. This peptide exhibits notable pro-angiogenic activity, as demonstrated by its ability to induce tube formation in human umbilical vein endothelial cells in vitro, an effect comparable to that of vascular endothelial growth factor. In a murine full-thickness dermal wound model, CW49 enhances wound healing by promoting the formation of granulation tissue and modulating epidermal thickness. Mechanistically, it upregulates the expression of key angiogenic proteins, including hypoxia-inducible factor-1 alpha, endothelial nitric oxide synthase, and inducible nitric oxide synthase, without significantly altering vascular endothelial growth factor levels. In addition to its angiogenic properties, CW49 displays anti-inflammatory activity by reducing the infiltration of macrophages and decreasing the levels of pro-inflammatory cytokines such as interleukin-6 and tumor necrosis factor-alpha in wound tissue. In vitro experiments with lipopolysaccharide-stimulated macrophages confirm that CW49 inhibits the secretion of these cytokines while promoting inducible nitric oxide synthase expression and nitric oxide production. This peptide does not significantly affect re-epithelialization or wound closure rates, suggesting its primary roles involve angiogenesis and modulation of the inflammatory response. CW49 represents a promising candidate for further investigation as a bioactive molecule with potential applications in tissue repair and regenerative biology.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Liu H, Duan Z, Tang J, Lv Q, Rong M, Lai R. A short peptide from frog skin accelerates diabetic wound healing. FEBS J. 2014 Oct;281(20):4633-43. doi: 10.1111/febs.12968. Epub 2014 Sep 8. Erratum in: FEBS J. 2026 Jan;293(1):318-319. doi: 10.1111/febs.70331. PMID: 25117795.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.