-
Product Name
CRBN antibody
- Documents
-
Description
CRBN Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue. Positive IF detected in COLO 320 cells, A375 cells. Positive WB detected in mouse heart tissue, A375 cells, COLO 320 cells, human brain tissue, human heart tissue, mouse liver tissue. Observed molecular weight by Western-blot: 53 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
cereblon antibody; CRBN antibody; DKFZp781K0715 antibody; MRT2A antibody; Protein cereblon antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of CRBN recombinant protein (Accession Number: NM_016302). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:109539(CRBN antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:109539(CRBN antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of COLO 320 cells using Catalog No:109539(CRBN Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561).
-
References
- Hohberger B, Enz R. Cereblon is expressed in the retina and binds to voltage-gated chloride channels. FEBS letters. 583(4):633-7. 2009.
- Lee KJ, Lee KM, Jo S, Kang KW, Park CS. Induction of cereblon by NF-E2-related factor 2 in neuroblastoma cells exposed to hypoxia-reoxygenation. Biochemical and biophysical research communications. 399(4):711-5. 2010.
- Greenberg AJ, Walters DK, Kumar SK, Vincent Rajkumar S, Jelinek DF. Responsiveness of cytogenetically discrete human myeloma cell lines to lenalidomide: lack of correlation with cereblon and interferon regulatory factor 4 expression levels. European journal of haematology. 91(6):504-13. 2013.
- Huang SY, Lin CW, Lin HH. Expression of cereblon protein assessed by immunohistochemicalstaining in myeloma cells is associated with superior response of thalidomide- and lenalidomide-based treatment, but not bortezomib-based treatment, in patients with multiple myeloma. Annals of hematology. 93(8):1371-80. 2014.
- Shortt J, Hsu AK, Martin BP. The drug vehicle and solvent N-methylpyrrolidone is an immunomodulator and antimyeloma compound. Cell reports. 7(4):1009-19. 2014.
- Gandhi AK, Mendy D, Waldman M. Measuring cereblon as a biomarker of response or resistance to lenalidomide and pomalidomide requires use of standardized reagents and understanding of gene complexity. British journal of haematology. 164(2):233-44. 2014.
- Ren Y, Wang M, Couto S. A Dual Color Immunohistochemistry Assay for Measurement of Cereblon in Multiple Myeloma Patient Samples. Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"