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COMMD5 antibody

Cat.#: 109444

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Product Information

  • Product Name
    COMMD5 antibody
  • Documents
  • Description
    COMMD5 Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in mouse kidney tissue, HEK-293 cells, human heart tissue, human stomach tissue, mouse heart tissue, mouse stomach tissue. Observed molecular weight by Western-blot: 25-28 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    COMM domain containing 5 antibody; COMMD5 antibody; FLJ13008 antibody; HCARG antibody; HT002 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of COMMD5 recombinant protein (Accession Number: NM_001081004). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

  • Validations

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:109444(COMMD5 antibody) at dilution of 1:1000

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:109444(COMMD5 antibody) at dilution of 1:1000

    IP Result of anti-COMMD5 (IP:Catalog No:109444, 4ug; Detection:Catalog No:109444 1:500) with mouse heart tissue lysate 3200ug.

    IP Result of anti-COMMD5 (IP:Catalog No:109444, 4ug; Detection:Catalog No:109444 1:500) with mouse heart tissue lysate 3200ug.

  • Background
    COMM domain containing 5 (COMMD5, synonyms: HCARG, HT002) is a hypertension-related calcium-regulated gene. COMMD5 is negatively regulated by extracellular calcium concentration, and its basal mRNA levels were higher in hypertensive animals. Tissue distribution of COMMD5 shows a preponderance in the heart, stomach, jejunum, kidney (tubular fraction), liver, and adrenal gland (mainly in the medulla). COMMD5 mRNA is significantly more expressed in adult than in fetal organs, and its levels are decreased in tumors and cancerous cell lines. COMMD5 protein has no transmembrane domain, but contains 67% -helix content, a calcium-binding site, four putative "leucine zipper" motifs, and a nuclear receptor-binding domain. At the subcellular level, COMMD5 shows a nuclear localization.
  • References
    • Chen BL, Yu J, Zeng ZR. Rosiglitazone suppresses gastric carcinogenesis by up-regulating HCaRG expression. Oncology reports. 20(5):1093-7. 2008.
    • Matsuda H, Lavoie JL, Gaboury L, Hamet P, Tremblay J. HCaRG accelerates tubular repair after ischemic kidney injury. Journal of the American Society of Nephrology : JASN. 22(11):2077-89. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"