CMV Cytomegalovirus Glycoprotein B (His Tag)

CMV Cytomegalovirus Glycoprotein B (His Tag)

• DataSheet
• Material Safety Data Sheets (MSDS)

Human cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 907-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.

GB; CMV GB; CYTOMEGALOVIRUS GLYCOPROTEIN B

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907) of human CMV gB (AAA45920.1, with furin cleavage site mutated from 'RTKR' to 'TTQT') was fused with a polyhistidine tag at the C-terminus.

CMV

The recombinant human CMV glycoprotein B (transmembrane domain deletion) comprises 818 amino acids and has a predicted molecular mass of 93 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant CMV gB is approximately 130-140 kDa due to glycosylation.

> 70 % as determined by SDS-PAGE

Measured by its ability to bind biotinylated Human CD209-Fc (Cat:501551) in functional ELISA.