CLE26 peptide

CLE26 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-RKVPRGPDPIHN-OH

H-Arg-Lys-Val-Pro-Arg-Gly-Pro-Asp-Pro-Ile-His-Asn-OH

12

95.45%

C₆₀H₁₀₀N₂₂O₁₆

1385.57

Synthetic

Powder

CLE26 is a peptide related to CLAVATA3/EMBRYO SURROUNDING REIGON. It has the same amino acid sequence as CLE25 (except for one amino acid substitution). In this study, it was found that CLE26 is associated with drought stress memory and promotes survival rate at the second dehydration event. CLE26 peptide signaling confers drought resistance in Arabidopsis in a cell-wall-modifying gene-dependent manner. Its transport changes in plants previously subjected to drought stress, and its function and transport depend on a cell-wall-modifying gene, XYLANASE1 (XYN1). The synthetic CLE26 peptide is well transported in both xyn1 and drought-pretreated wild-type plants but not in untreated wild-type plants. CLE26 has a similar structure to CLE25 and CLE45, differing from CLE25 only at position 5 and from CLE45 in positions 4 and 7.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Endo S, Fukuda H. A cell-wall-modifying gene-dependent CLE26 peptide signaling confers drought resistance in Arabidopsis. PNAS Nexus. 2024 Feb 1;3(2):pgae049. doi: 10.1093/pnasnexus/pgae049. PMID: 38352176; PMCID: PMC10863546.
• Diaz-Ardila HN, Gujas B, Wang Q, Moret B, Hardtke CS. pH-dependent CLE peptide perception permits phloem differentiation in Arabidopsis roots. Curr Biol. 2023 Feb 6;33(3):597-605.e3. doi: 10.1016/j.cub.2022.12.056. Epub 2023 Jan 23. PMID: 36693368.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.