CKAP4 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, 3T3-L1 cells, HeLa cells, rat kidney tissue. Positive IP detected in HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human liver tissue. Observed molecular weight by Western-blot: 63 kDa
ELISA, WB, IHC, IF, IP
Human,Mouse,Rat; other species not tested.
63 kDa membrane protein antibody; CKAP4 antibody; CLIMP 63 antibody; ERGIC 63 antibody; p63 antibody
This antibody was obtained by immunization of CKAP4 recombinant protein (Accession Number: NM_006825). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109328(CKAP4 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109328(CKAP4 antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109328(CKAP4 antibody) at dilution of 1:200 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CKAP4 antibody Catalog No:109328 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CKAP4 (IP:Catalog No:109328, 4ug; Detection:Catalog No:109328 1:1000) with HeLa cells lysate 2000ug.
CKAP4 represents cytoskeleton-associated protein 4
- Marini ES, Giampietri C, Petrungaro S. The endogenous caspase-8 inhibitor c-FLIPL regulates ER morphology and crosstalk with mitochondria. Cell death and differentiation. 22(7):1131-43. 2015.
- Khaminets A, Heinrich T, Mari M. Regulation of endoplasmic reticulum turnover by selective autophagy. Nature. 522(7556):354-8. 2015.
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