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Product Name
CKAP4 antibody
- Documents
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Description
CKAP4 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, 3T3-L1 cells, HeLa cells, rat kidney tissue. Positive IP detected in HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human liver tissue. Observed molecular weight by Western-blot: 63 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
63 kDa membrane protein antibody; CKAP4 antibody; CLIMP 63 antibody; ERGIC 63 antibody; p63 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CKAP4 recombinant protein (Accession Number: NM_006825). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109328(CKAP4 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109328(CKAP4 antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109328(CKAP4 antibody) at dilution of 1:200 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CKAP4 antibody Catalog No:109328 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CKAP4 (IP:Catalog No:109328, 4ug; Detection:Catalog No:109328 1:1000) with HeLa cells lysate 2000ug.
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Background
CKAP4 represents cytoskeleton-associated protein 4
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References
- Marini ES, Giampietri C, Petrungaro S. The endogenous caspase-8 inhibitor c-FLIPL regulates ER morphology and crosstalk with mitochondria. Cell death and differentiation. 22(7):1131-43. 2015.
- Khaminets A, Heinrich T, Mari M. Regulation of endoplasmic reticulum turnover by selective autophagy. Nature. 522(7556):354-8. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"