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  • CKAP4 antibody
  • CKAP4 antibody
  • CKAP4 antibody
  • CKAP4 antibody
  • CKAP4 antibody

CKAP4 antibody

Cat.#: 109328

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Special Price 239.0 USD

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Product Information

  • Product Name
    CKAP4 antibody
  • Documents
  • Description
    CKAP4 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, 3T3-L1 cells, HeLa cells, rat kidney tissue. Positive IP detected in HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human liver tissue. Observed molecular weight by Western-blot: 63 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    63 kDa membrane protein antibody; CKAP4 antibody; CLIMP 63 antibody; ERGIC 63 antibody; p63 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CKAP4 recombinant protein (Accession Number: BC082972). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109328(CKAP4 antibody) at dilution of 1:1000

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109328(CKAP4 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:109328(CKAP4 antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:109328(CKAP4 antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:109328(CKAP4 antibody) at dilution of 1:200 (under 40x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:109328(CKAP4 antibody) at dilution of 1:200 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using CKAP4 antibody Catalog No:109328 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using CKAP4 antibody Catalog No:109328 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-CKAP4 (IP:Catalog No:109328, 4ug; Detection:Catalog No:109328 1:1000) with HeLa cells lysate 2000ug.

    IP Result of anti-CKAP4 (IP:Catalog No:109328, 4ug; Detection:Catalog No:109328 1:1000) with HeLa cells lysate 2000ug.

  • Background
    CKAP4 represents cytoskeleton-associated protein 4
  • References
    • Marini ES, Giampietri C, Petrungaro S. The endogenous caspase-8 inhibitor c-FLIPL regulates ER morphology and crosstalk with mitochondria. Cell death and differentiation. 22(7):1131-43. 2015.
    • Khaminets A, Heinrich T, Mari M. Regulation of endoplasmic reticulum turnover by selective autophagy. Nature. 522(7556):354-8. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"