CGRRAGGSC peptide

CGRRAGGSC peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-CGRRAGGSC-NH2

H-Cys-Gly-Arg-Arg-Ala-Gly-Gly-Ser-Cys-NH2

9

95.98%

C₃₀H₅₄N₁₆O₁₀S₂

862.98

Synthetic

Powder

The peptide c(Cys-Gly-Arg-Arg-Ala-Gly-Gly-Ser-Cys)NH2 (denoted as CGRRAGGSC) is an artificially synthesized cyclic nonapeptide that binds to IL-11Rα, as confirmed by phage display technology. Using CGRRAGGSC, imaging and therapeutic analysis based on IL-11Rα expression level have been reported utilizing peptide conjugation with radioactive tracers such as ¹³¹I and ¹⁵³Sm. The specificity of Cy7-CGRRAGGSC in vivo was confirmed by a blocking experiment with unlabelled CGRRAGGSC and an excess of Cy7-CGRRAGGSC using a subcutaneous MCF-7 breast tumor xenograft. Cy7-CGRRAGGSC probe can enable target-specific imaging of tumors expressing IL-11Rα. Therefore, targeting IL-11Rα via optical imaging can be potentially used for the non-invasive and highly sensitive detection and characterization of IL-11Rα-expressing lesions, thus enhancing our ability to assess the effects of therapeutic interventions in the near future.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Li T, Sun J, Hu Y, Yang M, Shi H, Tang L. Near-infrared fluorescent labeled CGRRAGGSC peptides for optical imaging of IL-11Rα in athymic mice bearing tumor xenografts. J Biomed Res. 2019 Nov;33(6):391–7. doi: 10.7555/JBR.33.20180136. PMCID: PMC6891868.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.