CGN peptide

CGN peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-cgnhphlakyngt-OH, All D-type Amino acids

H-d-Cys-d-Gly-d-Asn-d-His-d-Pro-d-His-d-Leu-d-Ala-d-Lys-d-Tyr-d-Asn-d-Gly-d-Thr-OH

13

95.03%

C₆₀H₉₀N₂₀O₁₈S

1411.54

Synthetic

Powder

CGN is a peptide with the sequence d-CGNHPHLAKYNGT. It is the retro-inverso isomer of the TGN peptide. Previous study found that the TGN peptide facilitated polyethylene glycol-polylactide-polyglycolide (PEG-PLGA) nanoparticles across the blood-brain barrier (BBB), leading to significant higher brain accumulation. And CGN peptide exhibits higher brain targeting than that of TGN. It is used as a BBB targeting carrier.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Wang P, Zheng X, Guo Q, Yang P, Pang X, Qian K, Lu W, Zhang Q, Jiang X. Systemic delivery of BACE1 siRNA through neuron-targeted nanocomplexes for treatment of Alzheimer's disease. J Control Release. 2018 Jun 10;279:220-233. doi: 10.1016/j.jconrel.2018.04.034. Epub 2018 Apr 19. PMID: 29679667.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.