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Product Name
Rat CD55/DAF (Fc Tag) recombinant protein
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Description
CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 70-75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology.
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Protein name
Cd55 molecule
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Protein short names
DAF; GPI-DAF; RP11-357P18.1; TC; DAF-GPI; CR; CD55; CROM; AC111067.1; DAF1
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Uniprot ID
Q9Z0M0
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Gene Name
Cd55; DAF; Daf1; rCG_46364
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the rat CD55 (Q9Z0M0) (Met1-Ser371) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
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Protein Species
Rat
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Molecular weight
The recombinant rat CD55/Fc is a disulfide-linked homodimer. The reduced monomer comprises 578 amino acids and has a predicted molecular mass of 64.5 kDa. The apparent molecular mass of the protein is approximately 91 and 54 kDa in SDS-PAGE under reducing conditions.
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Purity
> 82 % as determined by SDS-PAGE
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Validations
Rat CD55 / DAF Protein (Fc Tag) SDS-PAGE
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