CD20 (188-196) peptide (TFA removed)

CD20 (188-196) peptide (TFA removed)

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-SLFLGILSV-OH

H-Ser-Leu-Phe-Leu-Gly-Ile-Leu-Ser-Val-OH

9

95.4%

C₄₆H₇₇N₉O₁₂

948.15

440962825

Synthetic

Powder

CD20 (188-196) plays a significant role in research focused on generating cytotoxic T lymphocytes and stimulating CTL responses against B cell-related diseases. The production of these specific T cells and IFN-γ is measured using ELISPOT assays. CD20 (188-196) has been recognized as a highly immunogenic peptide that is restricted by HLA-A2. Research findings suggest that CD20 (188-196) could be crucial in immunotherapeutic approaches, especially in developing vaccines against certain types of blood cancers. Additionally, CD20 (188-196) is utilized in cell therapy research as a target for T cell receptors. T cells are modified in vivo to express specific T cell receptors and these modified cells are then introduced into patients with B cell cancers. This modification enables the T cells to recognize and target malignant B cells, providing a therapeutic strategy for treating these cancers.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Jahn L, van der Steen DM, Hagedoorn RS, et al. Generation of CD20-specific TCRs for TCR gene therapy of CD20low B-cell malignancies insusceptible to CD20-targeting antibodies. Oncotarget. 2016;7(47):77021-77037. doi:10.18632/oncotarget.12778

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.